Title : High-Resolution Intracellular Recordings Using a Real-Time Computational Model of the Electrode
Authors : Romain Brette, Zuzanna Piwkowska, Cyril Monier, Michelle Rudolph, Julien Fournier, Manuel Levy, Yves Frégnac, Thierry Bal and Alain Destexhe
Year : 2008
Journal : Neuron
Volume : 59
Pages : 379-391
Supported by : SenseMaker
ntracellular recordings of neuronal membrane potential are a central tool in neurophysiology. In many situations, especially in vivo, the traditional lim- itation of such recordings is the high electrode resis- tance and capacitance, which may cause signi?cant measurement errors during current injection. We in- troduce a computer-aided technique, Active Elec- trode Compensation (AEC), based on a digital model of the electrode interfaced in real time with the elec- trophysiological setup. The characteristics of this model are ?rst estimated using white noise current injection. The electrode and membrane contribution are digitally separated, and the recording is then made by online subtraction of the electrode contribu- tion. Tests performed in vitro and in vivo demonstrate that AEC enables high-frequency recordings in de- manding conditions, such as injection of conduc- tance noise in dynamic-clamp mode, not feasible with a single high-resistance electrode until now. AEC should be particularly useful to characterize fast neuronal phenomena intracellularly in vivo.